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<h1 class="ltx_title ltx_title_section">Finding approximate positions of molecules</h1>

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<p class="ltx_p">Finding approximate positions of the molecules in the input images
follows the <a href="../filters/ui/Filters.html" title="" class="ltx_ref">image filtering and feature enhancement</a>
step. ThunderSTORM offers three algorithms for this purpose: detection
of local intensity maxima, non-maximum suppression, or calculation
of the centroid of connected components of segmented objects. All
three methods need a user-specified threshold defining an intensity
below which possible molecular detections will be rejected.</p>
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<span class="ltx_tag ltx_tag_itemize"><em class="ltx_emph">Input:</em></span> 
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<p class="ltx_p">A filtered image <img id="I1.ix1.p1.m1" class="ltx_Math" style="vertical-align:-2px" src="mi/mi2.png" width="18" height="16" alt="F"> of size <img id="I1.ix1.p1.m2" class="ltx_Math" style="vertical-align:-3px" src="mi/mi8.png" width="52" height="15" alt="m\times n"> pixels.</p>
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<span class="ltx_tag ltx_tag_itemize"><em class="ltx_emph">Output:</em></span> 
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<p class="ltx_p">A list of coordinates <img id="I1.ix2.p1.m1" class="ltx_Math" style="vertical-align:-10px" src="mi/mi7.png" width="104" height="32" alt="\left\{\left(\tilde{x}_{p},\tilde{y}_{p}\right)\right\}_{p=1}^{\tilde{P}}">
with approximate molecular positions.</p>
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<h2 class="ltx_title ltx_title_subsection">See also</h2>

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<span class="ltx_tag ltx_tag_itemize">•</span> 
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<p class="ltx_p"><a href="LocalMaximaDetector.html" title="" class="ltx_ref">Detection of local intensity maxima</a></p>
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</li>
<li id="I2.i2" class="ltx_item" style="list-style-type:none;">
<span class="ltx_tag ltx_tag_itemize">•</span> 
<div id="I2.i2.p1" class="ltx_para">
<p class="ltx_p"><a href="NonMaxSuppressionDetector.html" title="" class="ltx_ref">Non-maximum suppression</a></p>
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</li>
<li id="I2.i3" class="ltx_item" style="list-style-type:none;">
<span class="ltx_tag ltx_tag_itemize">•</span> 
<div id="I2.i3.p1" class="ltx_para">
<p class="ltx_p"><a href="CentroidOfConnectedComponentsDetector.html" title="" class="ltx_ref">Centroid of connected components</a></p>
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<li id="I2.i4" class="ltx_item" style="list-style-type:none;">
<span class="ltx_tag ltx_tag_itemize">•</span> 
<div id="I2.i4.p1" class="ltx_para">
<p class="ltx_p"><a href="Threshold.html" title="" class="ltx_ref">Threshold selection</a></p>
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